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Regulation of dimorphism in Histoplasma capsulatum by cyclic adenosine 3',5'-monophosphate
Journal of Bacteriology
|March 1, 1981
Abstract:
During temperature-induced transition of the dimorphic pathogenic fungus Histoplasma capsulatum from the single yeast cell form to the multicellular mycelial form, there was an increase in intracellular cyclic adenosine 3',5'-monophosphate (cAMP) levels as well as a striking accumulation of cAMP in the medium. cAMP levels also changed during the reverse mycelium-to-yeast transition.
Insights
During temperature changes, the pathogenic fungus Histoplasma capsulatum showed increased cyclic adenosine 3
Area of Science:
- Mycology
- Molecular Biology
- Biochemistry
Background:
- Histoplasma capsulatum is a dimorphic fungus.
- Dimorphism involves transitions between yeast and mycelial forms.
- These transitions are temperature-dependent and crucial for pathogenesis.
Purpose of the Study:
- To investigate the role of cyclic adenosine 3',5'-monophosphate (cAMP) during temperature-induced morphological transitions in Histoplasma capsulatum.
- To understand how intracellular and extracellular cAMP levels change during yeast-to-mycelium and mycelium-to-yeast transitions.
Main Methods:
- Culturing Histoplasma capsulatum at different temperatures to induce morphological changes.
- Measuring intracellular and extracellular concentrations of cyclic adenosine 3',5'-monophosphate (cAMP).
Main Results:
- A significant increase in intracellular cAMP was observed during the yeast-to-mycelium transition.
- Extracellular accumulation of cAMP was also noted during this transition.
- Changes in cAMP levels were also detected during the reverse mycelium-to-yeast transition.
Conclusions:
- Cyclic adenosine 3',5'-monophosphate (cAMP) plays a role in the temperature-induced dimorphic transition of Histoplasma capsulatum.
- Modulation of cAMP levels is associated with both yeast-to-mycelium and mycelium-to-yeast morphological changes.