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Mutation caused by human phagocytes.

S A Weitzman, T P Stossel

    Science (New York, N.Y.)
    |May 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Human immune cells, specifically phagocytes, can induce mutations in Salmonella typhimurium bacteria. This mutagenic effect is linked to the production of reactive oxygen metabolites by these immune cells.

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    Area of Science:

    • Microbiology
    • Immunology
    • Genetics

    Background:

    • The interaction between microbial pathogens and host immune cells is complex.
    • Understanding how the host immune system influences microbial genetic stability is crucial for infection control.

    Purpose of the Study:

    • To investigate the potential mutagenicity of human peripheral blood leukocytes on Salmonella typhimurium.
    • To identify the specific immune cell types and mechanisms responsible for inducing bacterial mutations.

    Main Methods:

    • Histidine-requiring Salmonella typhimurium TA100 mutants were co-incubated with human peripheral blood leukocytes.
    • Mutagenicity was assessed by the reversion rate of bacteria to histidine independence.
    • Different leukocyte preparations, including phagocyte-rich suspensions, lymphocytes, and leukocytes from a patient with chronic granulomatous disease, were tested.

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    Main Results:

    • A significantly higher rate of bacterial mutation (reversion to histidine independence) was observed when incubated with leukocytes compared to controls.
    • Phagocyte-rich leukocyte suspensions demonstrated mutagenic activity.
    • Heat-killed cells, lymphocytes, and leukocytes from a patient with impaired reactive oxygen metabolite production showed no mutagenic effect.

    Conclusions:

    • Human phagocytes possess the capacity to induce mutations in Salmonella typhimurium.
    • The production of reactive oxygen metabolites by phagocytes is likely responsible for this observed mutagenicity.
    • These findings highlight a novel mechanism by which host immune responses can impact microbial genetic integrity.