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[Comparison between 2 density gradients for separation of CFU].
Biomedicine / [Publiee Pour L'A.A.I.C.I.G.]
|November 1, 1980
Summary
Percoll and Ficoll-Metrizoate Sodium (Lymphoprep) density gradients yield similar colony-forming cells (CFU-C) per cell number. However, Percoll recovers a significantly higher absolute number of CFU-C from bone marrow samples.
Area of Science:
- Hematology
- Cell Biology
- Biotechnology
Background:
- Bone marrow stem cell isolation is crucial for research and clinical applications.
- Density gradient centrifugation is a common method for separating hematopoietic stem and progenitor cells.
- Comparing different density gradient media is essential for optimizing cell recovery.
Purpose of the Study:
- To compare the efficacy of Ficoll-Metrizoate Sodium (Lymphoprep) and Percoll density gradients for bone marrow cell separation.
- To evaluate the impact of these methods on colony-forming cell (CFU-C) recovery.
Main Methods:
- Bone marrow samples were separated using two density gradients: Ficoll-Metrizoate Sodium (Lymphoprep) and Percoll.
- Colony-forming cells (CFU-C) were quantified after isolation.
Main Results:
- No significant difference was observed in CFU-C yield when expressed per 2x10^5 bone marrow cells plated between the two methods.
- A significantly greater absolute number of recovered CFU-C was achieved using the Percoll gradient compared to Ficoll-Metrizoate Sodium.
Conclusions:
- Percoll density gradient centrifugation offers superior absolute recovery of colony-forming cells (CFU-C) from bone marrow compared to Ficoll-Metrizoate Sodium (Lymphoprep).
- While relative CFU-C yield is comparable, Percoll is advantageous for maximizing the absolute number of progenitor cells obtained.