The 5'-nucleotidase complex in sera from patients with gall stones--electrophoretic patterns and substrate...

Various purine and pyrimidine mononucleotides seem to be dephosphorylated by a 5'-nucleotidase complex composed of four fractions in sera from both patients with gall stones and from normal subjects. Agarose gel electrophoresis, with the addition of Triton X-100, demonstrated that the faster (F-) fraction of the enzyme complex and the slower (S-) fraction both consisted of two sub-fractions (termed alpha and beta). The enzyme complex showed a uniform substrate specificity pattern, which was independent of the absolute enzyme activity.