Effect of denervation on cyclic amp regulating enzymes in the rat gastrocnemius muscle

The activities of cyclic AMP regulatory enzymes (adenylate cyclase and phosphodiesterase) were studied in rat gastrocnemius muscle after denervation. Basal adenylate cyclase activity in whole homogenate increased progressively from the 3rd day reaching values more than 7-fold those of the controls 30 days after denervation. No significant differences between the contralateral and denervated muscle were detected as regards adenylate cyclase activation by catecholamines or NaF. Carbamylcholine was with effect. Cyclic AMP phosphodiesterase activity increased significantly at 3 days after denervation as was still significantly elevated at 60 days.