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Biological properties of plaque-size variants of Sendai virus
Abstract:
Large (RL)-and small (RS)-plaque variants of Sendai virus were isolated in culture of LLCMK2 cells in the presence of trypsin and their biological properties were determined. The RL variant was more virulent to mice than the RS variant. The RL variant had a higher growth rate than the RS variant in multiple-step growth in the presence of trypsin, but the two variants had an almost equal growth rate in its absence. Restoration of hemolytic activity in cleavage of the F protein of the RL variant were achieved by milder trypsin treatment than was needed for the RS variant.
Insights
Large (RL) and small (RS) plaque variants of Sendai virus showed different biological properties. The RL variant was more virulent and grew faster with trypsin, requiring milder treatment for hemolytic activity restoration.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- Sendai virus exhibits plaque variants with distinct biological characteristics.
- Understanding these variants is crucial for studying viral pathogenesis and host interactions.
Purpose of the Study:
- To isolate and characterize large (RL) and small (RS) plaque variants of Sendai virus.
- To determine the biological properties, including virulence, growth kinetics, and F protein activation, of these variants.
Main Methods:
- Isolation of Sendai virus RL and RS plaque variants in LLCMK2 cell cultures with trypsin.
- Assessment of biological properties: mouse virulence, multi-step growth curves (with and without trypsin), and hemolytic activity restoration via F protein cleavage.
Main Results:
- The RL variant demonstrated higher virulence in mice compared to the RS variant.
- RL variant exhibited a faster growth rate in the presence of trypsin, while growth rates were similar without trypsin.
- Milder trypsin treatment was sufficient to restore hemolytic activity through F protein cleavage in the RL variant compared to the RS variant.
Conclusions:
- Sendai virus plaque variants possess distinct biological properties influencing virulence and replication.
- Trypsin-dependent F protein cleavage plays a key role in the differential characteristics of RL and RS variants.
- These findings contribute to understanding Sendai virus variant-specific pathogenesis and host cell interactions.