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Isolation of Bordetella pertussis from swabs
Abstract:
Four varieties of swabs held in plastic containers and three swab transport media kits, all obtainable in the United Kingdom, were tested to assess their effectiveness in the isolation of Bordetella pertussis. The highest isolation rates up to 48 hours were obtained with Dacron swabs held in plastic containers. Swab-transport media kits proved unsatisfactory. B pertussis may have a stronger affinity to Dacron than to cotton-wool, and pernasal swabs should be made of Dacron to improve the recovery of B pertussis.
Insights
Dacron swabs in plastic containers best isolate Bordetella pertussis (whooping cough) for up to 48 hours. Swab-transport media kits were ineffective for pertussis diagnosis.
Area of Science:
- Microbiology
- Infectious Diseases
- Diagnostic Techniques
Background:
- Bordetella pertussis causes whooping cough, a significant public health concern.
- Accurate laboratory diagnosis is crucial for effective treatment and control of pertussis.
- Swab selection and transport methods can impact bacterial isolation rates.
Purpose of the Study:
- To evaluate the effectiveness of different swab types and transport media for Bordetella pertussis isolation.
- To identify optimal collection methods for improving B. pertussis recovery.
Main Methods:
- Tested four types of swabs in plastic containers and three swab transport media kits.
- Assessed isolation rates of B. pertussis over 48 hours.
- Compared recovery rates between different swab materials (Dacron vs. cotton-wool).
Main Results:
- Dacron swabs in plastic containers yielded the highest Bordetella pertussis isolation rates up to 48 hours.
- Swab-transport media kits were found to be unsatisfactory for B. pertussis recovery.
- B. pertussis demonstrated a stronger affinity for Dacron material compared to cotton-wool.
Conclusions:
- Dacron swabs in plastic containers are recommended for improved Bordetella pertussis isolation.
- Pernasal swabs made of Dacron material are suggested to enhance B. pertussis recovery.
- Swab-transport media kits should be reconsidered for pertussis diagnostics.