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In vitro method for evaluation of spermicides by hemolytic potency.
Fertility and Sterility
|August 1, 1981
Summary
Erythrocytes can predict spermicidal potency because Nonoxynol-9 affects them similarly to sperm. This allows for easier in vitro testing of vaginal spermicide formulations.
Area of Science:
- Reproductive biology
- Toxicology
Background:
- Nonoxynol-9 is a common spermicide.
- It immobilizes sperm and hemolyzes erythrocytes at similar concentrations.
Purpose of the Study:
- To evaluate erythrocytes as a model for predicting in vitro spermicidal potency of Nonoxynol-9.
- To enable extensive in vitro testing of vaginal spermicide formulations.
Main Methods:
- Comparing the effects of Nonoxynol-9 on erythrocytes and spermatozoa in vitro.
- Utilizing human or rabbit erythrocytes for testing.
Main Results:
- Nonoxynol-9 demonstrated analogous concentrations for immobilizing sperm and hemolyzing erythrocytes.
- Erythrocytes proved to be a viable alternative to spermatozoa for potency testing.
Conclusions:
- Erythrocytes can serve as a predictive model for in vitro spermicidal potency.
- This method facilitates more extensive testing of vaginal spermicide formulations due to erythrocyte availability.