Human papilloma virus type I purified from human genital warts

Human wart virus (HPV) was isolated from a pool of genital warts. The electrophoretic mobility of virion proteins was studied by SDS polyacrylamide gel electrophoresis and showed the same pattern as that obtained with HPV-1. The analysis of DNA after restriction enzyme digestion with the endonucleases Hind III and Hae III, and nucleic acid hybridization did not show any difference with HPV-1. The viral particles were agglutinated by anti-HPV-1 serum, as shown by electron microscopic particle agglutination test. Furthermore, the immunological properties of this virus were investigated with guinea pig antiserum. Serologically, no antigenic cross-reaction between common and genital wart viruses were shown by immunodiffusion and immunofluorescence tests, whereas cross-reactions were detected between plantar and genital wart viruses. These results allow to think that HPV-1 can induce plantar warts as well as genital warts.