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Related Experiment Videos

Nuclear progestin receptor-like component in human cervical tissue.

J A Holt, C A Pinkert, M A Lorincz

    The American Journal of Physiology
    |August 1, 1981
    PubMed
    Summary

    This study quantifies progestin-binding components in the human cervix, finding significant levels in both nuclear and cytoplasmic fractions. These findings are crucial for understanding progesterone

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    Human molecular genetics·2001

    Area of Science:

    • Endocrinology
    • Reproductive Biology
    • Molecular Pharmacology

    Background:

    • Progestin action is critical in human glandular cervix.
    • Understanding progestin receptor dynamics is key to reproductive health.

    Purpose of the Study:

    • To quantify progestin-binding components in the human cervix.
    • To characterize the properties of these progestin-binding sites.

    Main Methods:

    • Extraction of nuclear pellet with 0.5 M KCl.
    • Hydroxylapatite adsorption assay using [3H]R5020.
    • Sucrose density gradient ultracentrifugation for sedimentation analysis.

    Main Results:

    • Nuclear progestin-binding component levels were similar in follicular and luteal phases (930-995 fmol/g).
    • Cytoplasmic progestin receptor levels decreased in the luteal phase (2989 to 1861 fmol/g).
    • The nuclear progestin-binding component exhibited high affinity (Kd 2.6 X 10(-10) M), heat lability, and specificity.

    Conclusions:

    • The human cervix possesses significant progestin-binding components in both nuclear and cytoplasmic fractions.
    • These components display characteristics consistent with progestin receptors.
    • The findings contribute to understanding progesterone's role in cervical physiology.

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