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Epstein-Barr virus membrane antigens: characterization, distribution, and strain differences.

C M Edson, D A Thorley-Lawson

    Journal of Virology
    |July 1, 1981
    PubMed
    Summary

    Epstein-Barr virus (EBV) membrane antigens, including gp350 and gp220, are present on virus particles and producer cells. The B95-8 EBV strain is a structurally defective variant, with no observed protein pattern variations linked to disease states.

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    Tonsillar memory B cells, latently infected with Epstein-Barr virus, express the restricted pattern of latent genes previously found only in Epstein-Barr virus-associated tumors.

    Proceedings of the National Academy of Sciences of the United States of America·2000

    Area of Science:

    • Virology
    • Molecular Biology
    • Immunology

    Background:

    • Epstein-Barr virus (EBV) is a human herpesvirus associated with various diseases.
    • Previously identified EBV-associated membrane antigen polypeptides are located on producer cell lines.

    Purpose of the Study:

    • To determine the presence and localization of EBV-associated membrane antigen polypeptides on intact virus particles.
    • To characterize the structural integrity of different EBV isolates, including the B95-8 strain.

    Main Methods:

    • Subcellular fractionation to analyze antigen distribution within producer cells.
    • Immunoprecipitation to analyze EBV-associated polypeptides across 13 different EBV isolates.
    • Metabolic labeling with [3H]mannose and [3H]glucosamine, and tunicamycin sensitivity assays to identify glycoproteins.

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    Main Results:

    • EBV-associated membrane antigen polypeptides (gp350, gp220, p140, gp85) are present on intact EBV particles.
    • The B95-8 EBV strain and its derived cell lines exhibit a deficiency in the gp220 protein, suggesting it is a structurally defective variant.
    • gp350, gp220, and gp85 are identified as glycoproteins containing N-asparagine-linked glycosyl groups.

    Conclusions:

    • EBV membrane antigens are integral components of the virus particle.
    • The B95-8 EBV strain represents a structurally compromised variant.
    • No correlation was found between EBV protein patterns and specific disease states.