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Updated: Aug 17, 2026
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Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
Published on: April 4, 2011
A modified absorption-reduction method to detect virus-specific hemagglutination inhibiting and neutralizing IgM
Medical Microbiology and Immunology
|January 1, 1981
Abstract:
IgG antibodies are preferentially absorbed by protein A-coupled sepharose beads while most of the IgM and IgA antibodies remain in solution. Even relatively low amounts of virus-specific IgM antibodies can then be detected unequivocally by a decrease of the antibody titer following the treatment with reducing agents. Ethandithiol proved superior to 2-mercaptoethanol in combination with neutralization assays.
