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Pulmonary phosphatidic acid phosphohydrolase. Developmental patterns in rabbit lung.

P G Casola, F Possmayer

    Biochimica Et Biophysica Acta
    |August 24, 1981
    PubMed
    Summary

    Phosphatidic acid phosphohydrolase activity in rabbit lungs changes during development. Membrane-bound substrate activity peaks in late gestation, while aqueous substrate activity shows distinct molecular forms in adults.

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    Area of Science:

    • Biochemistry
    • Developmental Biology
    • Enzymology

    Background:

    • Phosphatidic acid phosphohydrolase (PAPh) is crucial for phospholipid metabolism.
    • Understanding PAPh developmental patterns in lung tissue is important for respiratory health.

    Purpose of the Study:

    • To investigate the developmental changes in PAPh activity in rabbit lungs.
    • To characterize PAPh activity using different phosphatidic acid substrates (aqueous and membrane-bound) and molecular forms.

    Main Methods:

    • Assessed PAPh activity in rabbit lung microsomes, homogenates, and cytosol during fetal development and in adults.
    • Utilized aqueously dispersed (PAaq) and membrane-bound (PAmb) phosphatidic acid as substrates.
    • Employed gel filtration and thermal denaturation to analyze enzyme properties.

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    Main Results:

    • PAmb-dependent PAPh activity increased in late gestation microsomes and homogenates, decreasing in adults.
    • PAaq-dependent activity showed a smaller gestational increase and adult decrease.
    • Adult cytosol PAPh revealed distinct molecular masses (150, 110 kDa for PAaq; 390, 240, 110 kDa for PAmb) not all present in fetal cytosol.
    • Thermal denaturation profiles differed between PAaq and PAmb activities and between fetal and adult stages.

    Conclusions:

    • PAPh activity and substrate preference exhibit significant developmental regulation in rabbit lungs.
    • Distinct molecular forms of PAPh are present in adult rabbit lung cytosol, suggesting developmental changes in enzyme composition.
    • These findings provide insights into the enzymatic regulation of phospholipid metabolism during lung development.