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A highly sensitive fluorimetric assay for pyridoxal phosphate phosphatase
Abstract:
A highly sensitive fluorimetric assay for pyridoxal phosphate phosphatase is described. The assay involves separation of the substrate and product by ion-exchange chromatography followed by treatment of pyridoxal with potassium cyanide under slightly alkaline conditions to form 4-pyridoxolactone, a highly fluorescent compound. Certain kinetic properties of the enzyme activities in human neutrophils are described.