High-performance liquid-chromatographic determination theophylline in plasma

Area of Science:

• Analytical Chemistry
• Pharmacology

Background:

• Therapeutic drug monitoring (TDM) is crucial for optimizing medication efficacy and safety.
• Theophylline is a bronchodilator used in managing respiratory diseases, requiring precise dosage adjustments.

Purpose of the Study:

• To develop and validate a high-performance liquid-chromatographic (HPLC) method for quantifying theophylline in human plasma.
• To establish a rapid, accurate, and reliable analytical technique for routine clinical use.

Main Methods:

• A reversed-phase HPLC method was employed.
• Plasma samples were deproteinized using acetonitrile.
• Theophylline detection was performed via UV absorption at 275 nm.

Main Results:

• The method demonstrated high sensitivity, accurately measuring theophylline down to 1.5 mg/L.
• Analysis time per sample was significantly reduced to approximately 7 minutes.
• No interference was observed from plasma components in samples from asthmatic patients.

Conclusions:

• The developed HPLC method offers a fast and accurate approach for theophylline plasma level determination.
• This technique is highly suitable for routine therapeutic drug monitoring in pediatric and adult populations.
• The method's efficiency and lack of interference support its clinical applicability.