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Related Experiment Videos

Clostridium difficile toxin as a confounding factor in enterovirus isolation.

N J Schmidt, H H Ho, M E Dondero

    Journal of Clinical Microbiology
    |December 1, 1980
    PubMed
    Summary

    Clostridium difficile toxin was identified as the cause of a unique cell-damaging effect observed during routine enterovirus testing. This finding impacts cell culture diagnostics and understanding of microbial interactions.

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    Area of Science:

    • Microbiology
    • Virology
    • Cell Biology

    Background:

    • Routine enterovirus isolation involves inoculating cell cultures with fecal specimens.
    • An unusual cytotoxic effect has been sporadically observed during this diagnostic process.

    Purpose of the Study:

    • To identify the causative agent of the peculiar cytotoxic effect observed in cell cultures during enterovirus isolation attempts.

    Main Methods:

    • Cell cultures were inoculated with fecal specimens.
    • Observation and characterization of a specific cytotoxic effect.

    Main Results:

    • The observed cytotoxic effect was definitively attributed to the toxin produced by Clostridium difficile.
    • This toxin interferes with standard enterovirus isolation procedures.

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    Conclusions:

    • Clostridium difficile toxin can mimic or interfere with viral cytopathic effects in cell cultures.
    • Awareness of this bacterial toxin is crucial for accurate enterovirus diagnosis and cell culture-based assays.