Biochemical characterization of the genetic variants of human phosphoglycolate phosphatase (PGP)

Phosphoglycolate phosphatase (PGP) exhibits a wide range of activities in normal human red cells. Analysis of blood from 57 individuals of known PGP phenotype revealed no correlation between enzyme activity, electrophoretic phenotype or 2,3-DPG concentration. Neither was there evidence of variation in Km, heat stability, pH optimum or molecular size among the various electrophoretic phenotypes. However, human red cell PGP exhibits high (0.24 mM) and low (0.05 mM) Km values for phosphoglycolate irrespective of phenotype and this requires further analysis.