[Localization of metallo-acceptor centers specifically binding divalent cations in myosin molecules]

The effects of DTNB and 4 M LiCl on ESR spectra saturation curves of myosin, spin-labelled at S1-SH groups in the presence of low concentrations of Mg2+ and Mn2+ ions have been studied. It has been shown that both DTNB and 4 M LiCl have taken off the influence of Mn2+ bound to high affinity metal binding sites of myosin on the character of saturation curves of spin-labelled protein. The results lead to a conclusion that high affinity metal binding sites of myosin are located in the LC2.light chains.