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Updated: Jul 21, 2026
11:11
Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
Published on: February 8, 2016
Abstract:
Supporting cells from 6- to 8-wk-old bovine calf testes were maintained in cell culture and were characterized morphologically and biochemically. Light and electron microscopy showed that the cultures consisted principally of supporting cells that were identified as epithelial cells with nucleolar vesicles. The bovine supporting cells in culture responded to follicle stimulating hormone with increased cAMP synthesis and enhanced incorporation of [3H]leucine into secreted proteins. No androgen binding protein could be detected in the medium.
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01:41Spermatogenesis
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
01:21Cell Culture
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The spermatogenic cells, responsible for producing sperm, are...
The spermatogenic cells, responsible for producing sperm, are...