Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Aging of the erythrocyte XIII. Decrease in nitroxide reducing capacity.

G Bartosz, K Gwozdzinski, J Wagner

    Biomedicine / [Publiee Pour L'A.A.I.C.I.G.]
    |November 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Flavanols protect the yeast Saccharomyces cerevisiae against heating and freezing/thawing injury.

    Journal of applied microbiology·2018
    Same author

    Association of five SNPs with human hair colour in the Polish population.

    Homo : internationale Zeitschrift fur die vergleichende Forschung am Menschen·2017
    Same author

    Endothelial glycocalyx integrity is preserved in young, healthy men during a single bout of strenuous physical exercise.

    Physiological research·2015
    Same author

    Nitroxides prevent protein glycoxidation in vitro.

    Free radical research·2014
    Same author

    Posttranslational protein modifications by reactive nitrogen and chlorine species and strategies for their prevention and elimination.

    Free radical research·2014
    Same author

    Cellular redox homeostasis in endothelial cells treated with nonmodified and Fenton-modified nanodiamond powders.

    Biotechnology and applied biochemistry·2014

    Researchers developed a new method to measure the nitroxide-reducing capacity (NRC) in cells. This study found that cellular NRC declines with age in bovine erythrocytes.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Spectroscopy

    Background:

    • Cellular redox balance is crucial for cell function.
    • Nitroxide stable free radicals are sensitive probes of cellular reduction potential.

    Purpose of the Study:

    • To establish a novel method for quantifying nitroxide-reducing capacity (NRC) in cellular systems.
    • To investigate the changes in NRC during erythrocyte aging.

    Main Methods:

    • Electron Spin Resonance (ESR) spectroscopy was employed to measure the reduction of a nitroxide stable free radical.
    • The method was applied to cell suspensions, including erythrocytes.

    Main Results:

    • A reliable method for determining cellular NRC was established.

    Related Experiment Videos

  • A significant decrease in NRC was observed in older fractions of bovine erythrocytes compared to younger ones.
  • This indicates a decline in cellular reducing capacity during in vivo aging.

    • Conclusions:

    • The proposed ESR-based method is effective for assessing cellular NRC.
    • Erythrocyte aging in vivo is associated with a diminished nitroxide-reducing capacity.
    • This finding has implications for understanding age-related cellular changes.