Nucleotide sequence of the Akv env gene

Area of Science:

• Virology
• Molecular Biology
• Genetics

Background:

• Murine retroviruses, such as Akv, are significant models for studying viral replication and pathogenesis.
• The envelope (env) gene encodes surface glycoproteins crucial for viral entry and assembly.

Purpose of the Study:

• To determine the nucleotide sequence of the Akv murine retrovirus env gene.
• To analyze the predicted amino acid sequence and structural features of the env gene products.
• To compare the env gene sequence with those of leukemogenic viruses to understand structural differences.

Main Methods:

• Nucleotide sequencing of a molecular clone of the Akv provirus.
• Deduction of amino acid sequences from nucleotide sequences.
• Analysis of predicted protein structures, including glycosylation sites and membrane association regions.
• Comparison of RNase T1-resistant oligonucleotides.

Main Results:

• The 2,191-nucleotide env gene sequence was determined, encoding a 638-amino acid precursor for gp70 and p15E.
• Structural analysis predicted membrane localization for gp70 and p15E, with specific regions for membrane fusion and association.
• Cleavage sites and potential interactions with virion cores during budding were identified.
• Comparison with leukemogenic viruses revealed differences in the primary structure of env gene products.

Conclusions:

• The study provides a detailed structural map of the Akv retrovirus env gene product.
• Predicted membrane topology and cleavage sites offer insights into viral assembly and entry mechanisms.
• Sequence comparisons highlight potential structural variations contributing to the leukemogenicity of related viruses.