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Somatic variants of murine immunoglobulin lambda light chains.

A L Bothwell, M Paskind, M Reth

    Nature
    |July 22, 1982
    PubMed
    Summary
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    Somatic point mutations in lambda genes, unlike earlier findings for kappa genes, occur in both framework and complementarity-determining regions (CDRs). This research clarifies the sites of genetic variation in antibody light chains.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Genetics

    Background:

    • Somatic point mutation of germ-line variable (V) region genes was first evidenced in murine lambda light chains.
    • Previous studies suggested preferential somatic mutation in complementarity-determining regions (CDRs) of lambda genes.
    • Contrasting evidence from kappa genes indicated mutation in both framework and CDR residues.

    Purpose of the Study:

    • To investigate the discrepancy in somatic mutation sites between lambda and kappa genes.
    • To determine the nucleotide sequences of specific lambda genes to clarify mutation patterns.

    Main Methods:

    • DNA sequence analysis of rearranged functional lambda 1 and lambda 2 genes from hybridomas and myeloma cells.
    • Comparison of nucleotide sequences to identify somatic mutations.

    Related Experiment Videos

    Main Results:

    • Nucleotide sequence determination of two lambda 1 genes and one lambda 2 gene was performed.
    • Results demonstrated that somatic mutations in lambda genes can occur in both framework and CDR residues.
    • This finding resolves the apparent discrepancy with earlier observations.

    Conclusions:

    • Somatic mutation in lambda genes is not restricted to CDRs and also affects framework residues.
    • This study provides a more comprehensive understanding of V(D)J recombination and antibody diversification mechanisms.
    • The findings contribute to the broader field of adaptive immunity and genetic variation.