[Method of determining the biochemical behavior of clostridia]

A fast and readily applicable method is suggested for the determination of the biochemical reactions of the spore producing anaerobic organisms. A pepton coloid medium with the addition of 0.15 per cent agar-agar was used as a basic nutrient medium. The cultivation of bacteria did not require any special conditions. Reading of the results was carried out with the addition of 0.1 per cent phenol red after 48 hours of incubation of the cultures. This method showed a number of advantages over the methods used so far.