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Changes in hepatic microsomal membrane fluidity with age.

H J Armbrecht, L S Birnbaum, T V Zenser

    Experimental Gerontology
    |January 1, 1982
    PubMed
    Summary
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    Aging increases the fluidity of rat liver microsomal membranes, indicated by changes in membrane order parameter. This age-related increase in fluidity may explain altered enzyme activities.

    Area of Science:

    • Biochemistry
    • Gerontology
    • Membrane Biology

    Background:

    • Mixed function oxidase (MFO) enzymatic activities in rat hepatic microsomal membranes change with age.
    • Protein components of the MFO system do not appear to change with age, suggesting a role for the lipid component.

    Purpose of the Study:

    • To investigate age-related changes in the fluidity of the lipid component of rat hepatic microsomal membranes.
    • To determine if altered membrane fluidity correlates with changes in MFO activity.

    Main Methods:

    • Hepatic microsomes were isolated from male CFN rats of 3, 12, and 26 months of age.
    • Membrane fluidity was measured using electron paramagnetic resonance (EPR) with nitroxide stearic acid spin labels.
    • The order parameter (S) was calculated to quantify membrane fluidity.

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    Main Results:

    • A decrease in the order parameter (S) with age was observed, indicating increased membrane fluidity.
    • Membrane fluidity increased from 0.586 (3 mo) to 0.569 (26 mo) at 30°C.
    • A phase transition at 24°C was observed in membranes from 26-month-old rats, but not in 3-month-old rats.

    Conclusions:

    • Rat hepatic microsomal membrane fluidity increases with age.
    • This age-dependent increase in membrane fluidity may contribute to observed age-related changes in MFO activities.