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Published on: April 26, 2011
A micromethod for the determination of ceramide
Journal of Neuroscience Methods
|May 1, 1982
Abstract:
An enzymatic micromethod for the determination of ceramide is presented. The enzyme, E. coli diglyceride kinase was used to phosphorylate ceramide, as well as diglyceride with high specific activity gamma-[32P]ATP, and the two products are differentiated by their alkali stability. This method was applied to the detection of endogenous phospholipases and sphingolipases in several membrane systems and could have wide application.
