[Immunoenzyme analysis. The comparative characteristics of 2 methods of obtaining a hormone and enzyme complex]

Two methods for protein conjugation are routinely used in biochemical studies. One of them is based on the binding of free protein amino groups via functionally active elements of glutaraldehyde, whereas the other one makes use of covalent binding of proteins; carbohydrate groups of one of these proteins are preoxidized with sodium periodate to form free aldehyde groups. The possibility of employing these two methods for enzyme immunologic analysis of hypophyseal hormones was examplified by obtaining the complex of the ox growth hormone with horse radish peroxidase.