Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Solid-phase colorimetric determination of potassium.

S C Charlton, R L Fleming, A Zipp

    Clinical Chemistry
    |September 1, 1982
    PubMed
    Summary

    This study presents a new dye-binding method for quantifying potassium levels using a plasticized polyvinyl chloride film and valinomycin ionophore. The method is accurate in aqueous and serum solutions, unaffected by sodium concentrations.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    What I've learned from 135 patient deaths.

    Medical economics·1997
    Same author

    The extreme regressive reaction of a psychopath.

    The Psychiatric quarterly·1996
    Same author

    Living arrangements and nutrient intakes of healthy women age 65 and older: a study in Manhattan, Kansas.

    Journal of nutrition for the elderly·1992
    Same author

    Dual 24-hour feeding response to 2DG in rats: daytime increase and nighttime decrease.

    Physiology & behavior·1989
    Same author

    Dry-reagent strips used for determination of theophylline in serum.

    Clinical chemistry·1985
    Same author

    A comparison between chlordiazepoxide and CL 218,872--a synthetic nonbenzodiazepine ligand for benzodiazepine receptors on spontaneous locomotor activity in rats.

    Psychopharmacology·1985

    Area of Science:

    • Analytical Chemistry
    • Biophysical Chemistry

    Background:

    • Accurate potassium (K+) quantification is crucial in biological and chemical analyses.
    • Existing methods may face limitations in complex matrices like serum.
    • Development of novel, selective K+ detection methods is ongoing.

    Purpose of the Study:

    • To develop and validate a novel dye-binding method for quantitative potassium ion determination.
    • To assess the method's applicability in both aqueous and serum-based solutions.
    • To investigate potential interferences and optimize the assay components.

    Main Methods:

    • A nonpolar organic film of plasticized polyvinyl chloride incorporating the ionophore valinomycin was utilized.
    • The film was incubated with solutions containing potassium ions and the anionic dye erythrosin B.
    • Quantification of retained erythrosin B by absorbance or reflectance measurements after washing.

    Main Results:

    • A direct correlation was established between retained erythrosin B and potassium ion concentration.
    • The dye-binding method demonstrated quantitative accuracy for potassium in aqueous and serum samples.
    • No significant interference from physiological sodium concentrations was observed.
    • Several plasticizers, anionic dyes, and ionophores proved effective.

    Conclusions:

    • The valinomycin-based dye-binding method offers a reliable and selective approach for potassium quantification.
    • This technique is suitable for diverse sample types, including biological fluids.
    • Anion binding appears to be a surface-restricted phenomenon within the film.

    Related Experiment Videos