Regional distribution of perchloric acid soluble proteins during the development of rat brain

The regional contents of perchloric acid (PCA)-soluble proteins were determined during the development of rat brain. The soluble proteins in cortex, mesencephalon and medulla oblongata decreased gradually to adult age, whereas the levels in cerebellum increased significantly 12 days after birth followed by a decline to adult age. The developmental changes in the soluble proteins in retina, which showed the highest content of these proteins, were different from that of the other regions. Changes in the staining intensities of the main protein bands following SDS-gel electrophoresis were observed during the development of all regions. The patterns of the soluble proteins from retina and cerebellum were less complex than those of the other brain regions and liver and resembled that of the chromosomal high-mobility group proteins and H 1 histones.