Chromosomal location of a Bacillus subtilis DNA fragment uniquely transcribed by sigma-28-containing RNA polymerase

A fragment of the Bacillus subtilis chromosome containing a gene whose transcription is dependent on sigma-28-containing RNA polymerase has been genetically mapped by means of an integrable plasmid. This gene resides on the chromosome adjacent to the stage 0 sporulation locus spo0E between metC and citL. The gene was insertionally inactivated by cloning an internal EcoRI-HindIII fragment in the integrable plasmid pJH101 and by inserting the plasmid into the chromosome by transformation. Transformants bearing an inactivated gene were indifferent to this inactivation for both growth and sporulation.