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Alterations in plasma-membrane functions after poliovirus infection
Bioscience Reports
|August 1, 1982
Summary
Poliovirus infection rapidly alters HeLa cell membranes, decreasing fluorescence polarization and increasing (Na+/K+)ATPase activity. This leads to reduced cell volume and a rise in host protein synthesis inhibition.
Area of Science:
- Cell Biology
- Virology
- Biochemistry
Background:
- Poliovirus infection triggers significant cellular changes in host cells.
- Understanding early viral-induced host cell modifications is crucial for antiviral development.
Purpose of the Study:
- To investigate the immediate effects of poliovirus on HeLa cell membrane properties and intracellular environment.
- To characterize early molecular and biophysical changes following poliovirus exposure.
Main Methods:
- Measurement of 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence polarization in HeLa cells.
- Assay of (Na+/K+)ATPase activity in isolated plasma membrane fractions.
- Quantification of cell volume and intracellular inhibitor concentration.
Main Results:
- A rapid decrease in DPH fluorescence polarization was observed within minutes post-infection.
- Enhanced (Na+/K+)ATPase activity, decreased cell volume, and increased inhibitor levels were detected within one hour.
- These findings indicate swift alterations in membrane dynamics and cellular homeostasis.
Conclusions:
- Poliovirus infection induces rapid biophysical and biochemical changes in HeLa cells.
- Early cellular responses include membrane alterations and the activation of host protein synthesis inhibition pathways.
- These early events are critical for viral replication and host cell manipulation.