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IS3 can function as a mobile promoter in E. coli.

D Charlier, J Piette, N Glansdorff

    Nucleic Acids Research
    |October 11, 1982
    PubMed
    Summary
    This summary is machine-generated.

    The insertion of an IS3 element near the E. coli argE gene activates its expression. This activation stems from an outward promoter on the IS3 element, functioning as a mobile promoter.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • The E. coli argE gene's expression can be influenced by insertion sequences.
    • Previous work indicated IS3 element insertion activates argE expression.

    Purpose of the Study:

    • To identify the mechanism by which the IS3 element activates the E. coli argE gene.
    • To pinpoint the location and nature of the promoter responsible for argE activation by IS3.

    Main Methods:

    • DNA sequencing to determine the precise insertion site of the IS3 element.
    • S1 nuclease mapping using in vivo transcribed RNA to locate the promoter.

    Main Results:

    • The IS3 element contains an outward promoter responsible for activating argE transcription.
    • This promoter is located on IS3 outside its inverted repeat regions.
    • The exact insertion site of IS3 was confirmed via DNA sequencing.

    Conclusions:

    • The IS3 insertion sequence acts as a mobile promoter, driving the transcription of the adjacent E. coli argE gene.
    • The identified promoter on IS3 is distinct from the element's inverted repeat regions.