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Related Experiment Videos

A poxvirus antigen associated with pathogenicity for rabbits.

C J Rondle, K R Dumbell

    The Journal of Hygiene
    |December 1, 1982
    PubMed
    Summary

    White pock variants of cowpox virus lack a specific antigen ("d" substance) and cause papular lesions in rabbits. Recombinant virus studies suggest genes for "d" substance and rabbit skin pathogenicity are closely linked on the poxvirus genome.

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    Area of Science:

    • Virology
    • Molecular Biology
    • Genetics

    Background:

    • Cowpox virus (CPXV) exhibits variants, including white pock variants, which differ in pathogenicity.
    • White pock CPXV variants fail to produce a specific precipitating antigen, termed "d" substance.
    • Previous research established a link between CPXV variants and antigen production.

    Purpose of the Study:

    • To investigate the presence of "d" substance in various poxviruses.
    • To determine the genetic linkage between "d" substance production and rabbit skin pathogenicity.
    • To analyze recombinant poxviruses for antigen production and pathogenicity.

    Main Methods:

    • Analysis of soluble antigen preparations from rabbitpox virus, vaccinia virus, and variola virus for "d" substance.
    • Testing of recombinant poxviruses derived from variola, cowpox, and rabbitpox viruses.
    • Comparison of recombinant virus "d" substance production with their previously recorded intradermal effects in rabbits.

    Main Results:

    • "d" substance was demonstrable in rabbitpox and neurovirulent vaccinia virus antigens, but not in variola virus antigens.
    • Recombinant viruses showed specific patterns of "d" substance production.
    • Results were correlated with known rabbit skin pathogenicity of these recombinant viruses.

    Conclusions:

    • Functional genes for "d" substance production and rabbit skin pathogenicity are closely linked on the poxvirus genome.
    • While linked, the interdependence of these two functions remains inconclusive.
    • This study provides insights into poxvirus genetics and antigen expression.

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