Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Kilo-sequencing: an ordered strategy for rapid DNA sequence data acquisition.

W M Barnes, M Bevan

    Nucleic Acids Research
    |January 25, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cross-sectional study of the prevalence, causes and management of hospital-onset diarrhoea.

    The Journal of hospital infection·2019
    Same author

    The interaction of auxin and cytokinin in the induction of phenylalanine ammonia-lyase in suspension cultures of Phaseolus vulgaris.

    Planta·2013
    Same author

    Subculture-induced protein synthesis in tissue cultures of Glycine max and Phaseolus vulgaris.

    Planta·2013
    Same author

    Some rapid effects of synthetic auxins on mRNA levels in cultured plant cells.

    Planta·2013
    Same author

    Genetic transformation of apple (Malus pumila Mill.) using a disarmed Ti-binary vector.

    Plant cell reports·2013
    Same author

    Genetic transformation in two potato cultivars with T-DNA from disarmed Agrobacterium.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
    Same journal

    Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

    Nucleic acids research·2026
    Same journal

    Correction to 'Differentiable partition function calculation for RNA'.

    Nucleic acids research·2026
    Same journal

    Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

    Nucleic acids research·2026
    Same journal

    Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

    Nucleic acids research·2026
    Same journal

    Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

    Nucleic acids research·2026
    Same journal

    Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

    Nucleic acids research·2026
    See all related articles

    This study introduces a method for rapid, ordered DNA sequencing using M13 phage vectors. It generates targeted DNA deletions for efficient nucleotide sequence acquisition from specific DNA regions.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biotechnology

    Background:

    • Traditional DNA sequencing methods can be time-consuming and lack order.
    • M13 phage vectors offer convenient DNA template handling.
    • Efficient acquisition of nucleotide sequence data is crucial for genetic research.

    Purpose of the Study:

    • To develop a strategy for rapid, ordered DNA sequence acquisition.
    • To retain the advantages of the dideoxy sequencing method with M13 phage DNA templates.
    • To enable sequencing of target DNA fragments ranging from 3 to 14 kilobases.

    Main Methods:

    • Utilizing M13 vectors to carry target DNA (3-14 kb).
    • Employing an in vitro procedure involving random and specific DNA cutting to create deletions.

    Related Experiment Videos

  • Purifying phage with desired deletion sizes via agarose gel electrophoresis.

    • Main Results:

    • Generated thousands of deletions starting from a unique restriction site adjacent to the sequencing primer.
    • Successfully obtained nucleotide sequence data from specific kilobase regions of target DNA.
    • Demonstrated that phage from the same gel location yield sequence data from the same DNA kilobase.

    Conclusions:

    • The described strategy allows for rapid and ordered DNA sequencing.
    • This method preserves the conveniences of dideoxy sequencing with M13 phage templates.
    • It provides a reliable approach for targeted nucleotide sequence acquisition from large DNA fragments.