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Effect of pulsed ultrasound on human erythrocytes in vitro
Pulsed echo sonication of whole blood affects the O2-Hb dissociation curve and produces modifications of the erythrocyte membrane, whereas Hb remain unchanged. After sonication ATP-ase (Na+K+ dependent) activity and agglutination disappear. Sonication detaches erythrocytes-membrane antigens which are found in the supernatant, this effect is reversible. AB antigens detached by sonication were transferred to sonicated O group erythrocytes. Examination by transmission electron microscopy suggested that the glycoproteic cup or glycocalyx of the red cells was removed as a result of sonication.
Pulsed echo sonication of whole blood affects the O2-Hb dissociation curve and produces modifications of the erythrocyte membrane, whereas Hb remain unchanged. After sonication ATP-ase (Na+K+ dependent) activity and agglutination disappear. Sonication detaches erythrocytes-membrane antigens which are found in the supernatant, this effect is reversible. AB antigens detached by sonication were transferred to sonicated O group erythrocytes. Examination by transmission electron microscopy suggested that the glycoproteic cup or glycocalyx of the red cells was removed as a result of sonication.