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Updated: Oct 20, 2026

Isolation, Fixation, and Immunofluorescence Imaging of Mouse Adrenal Glands
08:37

Isolation, Fixation, and Immunofluorescence Imaging of Mouse Adrenal Glands

Published on: October 2, 2018

[Immunology of beta-adrenergic receptors: a working model of a hormone-mimicking internal image]

A D Strosberg, P O Couraud, C Delavier-Klutchko

    Annales D'Immunologie
    |September 1, 1982
    PubMed

    Two types of antibodies have been prepared against beta-adrenergic receptors: (1) antibodies against receptor, purified from turkey erythrocyte membranes by affinity chromatography on alprenolol sepharose, and (2) antiidiotypic antibodies raised against the anti-alprenolol immunoglobulins. Both types of antibodies specifically bind to cells which possess beta-adrenergic receptor and mimick the biological effect of the catecholamine hormone: they stimulate basal adenylate-cyclase and enhance adenylate-cyclase activation by catecholamines. The antiidiotypic antibodies only compete with (--)-3H-dihydroalprenolol for binding to the beta-adrenergic receptors on purified turkey erythrocyte membranes. The use of these antibodies as tools for the study of the mechanism of the beta-adrenergic receptor-cyclase complex is discussed.

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