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Related Experiment Videos

Mutagenic virus replication in human tumor cells.

L E Bockstahler, C D Lytle, A S Lubiniecki

    Cancer Letters
    |November 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

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    Human tumor cells increase herpes simplex virus mutation rates. Virus grown in tumor cell lines showed higher iododeoxycytidine resistance, indicating increased mutagenicity compared to normal cells.

    Area of Science:

    • Virology
    • Cancer Cell Biology
    • Genetics

    Background:

    • Herpes simplex virus (HSV) is a common human pathogen.
    • Understanding viral replication and mutation is crucial for disease control.
    • Tumor cells may possess unique properties influencing viral behavior.

    Purpose of the Study:

    • To investigate the mutagenic potential of herpes simplex virus replication in human tumor cells versus normal cells.
    • To determine if human tumor cell lines enhance viral mutation rates.

    Main Methods:

    • Herpes simplex virus was cultured in various human tumor and normal cell lines.
    • Progeny virus was tested for resistance to iododeoxycytidine, a marker for viral genome mutations.
    • Mutation frequencies were compared between virus grown in tumor cells and normal cells.

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    Main Results:

    • Virus propagated in 4 out of 5 human tumor cell lines exhibited significantly higher fractions of iododeoxycytidine resistance compared to virus grown in 7 normal human skin cell lines.
    • In two osteosarcoma patient cases, tumor cells showed enhanced mutagenesis, while their normal skin fibroblasts did not.
    • These findings suggest a mutagenic effect of certain tumor cell environments on herpes simplex virus.

    Conclusions:

    • Some human tumor cell lines can alter the herpesvirus replication process, leading to increased mutagenicity.
    • This phenomenon may have implications for understanding virus-tumor interactions and potential therapeutic strategies.