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Nucleotide sequence of Rous sarcoma virus.

D E Schwartz, R Tizard, W Gilbert

|March 1, 1983

Summary

We sequenced the Prague C strain of Rous sarcoma virus (RSV), revealing gene locations and overlaps. A specific DNA element (E) differs in position between RSV strains, suggesting ancestral duplication and deletion.

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Area of Science:

Virology
Molecular Biology
Genomics

Background:

Rous sarcoma virus (RSV) is a retrovirus known to cause tumors in chickens.
Understanding RSV genome organization is crucial for studying viral replication and oncogenesis.
Previous studies have characterized some RSV genes but lacked complete genomic sequence data for certain strains.

Purpose of the Study:

To determine the complete nucleotide sequence of the Prague C (Pr-C) strain of RSV.
To identify and map the coding regions for the gag, pol, env, and src genes within the Pr-C RSV genome.
To compare the genomic sequences flanking the src gene in Pr-C and Schmidt-Ruppin (SR-A) RSV strains to understand evolutionary relationships.

Main Methods:

Whole-genome sequencing of the Prague C strain of Rous sarcoma virus.
Bioinformatic analysis to identify open reading frames and assign gene functions (gag, pol, env, src).
Comparative sequence analysis between Pr-C and SR-A RSV strains, focusing on regions surrounding the src gene.

Main Results:

The complete 9312 nucleotide sequence of the Pr-C RSV strain was determined.
Coding regions for gag, pol, env, and src genes were assigned, with gag in a different reading frame than pol.
The pol and env genes were found to overlap.
A 153-nucleotide element (E) was located 3' to the src gene in Pr-C RSV and 5' to the src gene in SR-A RSV.

Conclusions:

The Pr-C RSV genome sequence provides a comprehensive resource for retroviral research.
The positional difference of element E between RSV strains suggests a mechanism of gene duplication and deletion during evolution.
This finding sheds light on the genomic plasticity and evolutionary history of Rous sarcoma virus.

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