Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease in the average size of replicons in a Werner syndrome cell line by Simian virus 40 infection.

F Hanaoka, F Takeuchi, T Matsumura

    Experimental Cell Research
    |April 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Lean non-alcoholic fatty liver disease (lean NAFLD): characteristics, metabolic outcomes and risk factors from a 7-year prospective, community cohort study from Sri Lanka.

    Hepatology international·2018
    Same author

    Online control of a humanoid robot through hand movement imagination using CSP and ECoG based features.

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2016
    Same author

    Distinction of individual finger responses in somatosensory cortex using ECoG high-gamma activation mapping.

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2016
    Same author

    Genetic variants of Kudoa septempunctata (Myxozoa: Multivalvulida), a flounder parasite causing foodborne disease.

    Journal of fish diseases·2015
    Same author

    A Comparison between Revised NCEP ATP III and IDF Definitions in Diagnosing Metabolic Syndrome in an Urban Sri Lankan Population: The Ragama Health Study.

    ISRN endocrinology·2013
    Same author

    The burden of diabetes mellitus and impaired fasting glucose in an urban population of Sri Lanka.

    Diabetic medicine : a journal of the British Diabetic Association·2012
    Same journal

    Early-Life Cardiovascular Programming by Maternal Protein Restriction: Electrophysiological, Structural, and Molecular Alterations in Female Rats.

    Experimental cell research·2026
    Same journal

    The AP2α/PDHA1 signaling pathway promotes lung cancer progression by mediating aerobic glycolysis and inhibiting cuproptosis.

    Experimental cell research·2026
    Same journal

    Distributed transcription activity in DLX proteins defies conventional mapping of a transcription activation domain.

    Experimental cell research·2026
    Same journal

    Extracellular release of cytotoxic aggregates from HSV-1-replicating SH-SY5Y cells: involvement of alpha-synuclein and poly-ubiquitin conjugates.

    Experimental cell research·2026
    Same journal

    MiR-1281 downregulates LMX1B to inhibit gastric cancer development.

    Experimental cell research·2026
    Same journal

    Corrigendum to "Heat shock protein 90β stabilizes focal adhesion kinase and enhances cell migration and invasion in breast cancer cells" [Exp. Cell Res., 326, 1, 1 August 2014, Pages 78-89].

    Experimental cell research·2026
    See all related articles

    Werner syndrome (WS) fibroblasts show longer distances between DNA replication origins. Simian Virus 40 infection normalizes these distances in WS cells, suggesting a role for viral infection in DNA replication regulation.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Virology

    Background:

    • Werner syndrome (WS) is a premature aging disorder linked to DNA repair defects.
    • Understanding DNA replication in WS cells is crucial for disease mechanisms.
    • Simian Virus 40 (SV40) is a model virus used to study DNA replication.

    Purpose of the Study:

    • To investigate DNA replication dynamics in Werner syndrome (WS) fibroblasts.
    • To compare replicon initiation site distances and DNA chain elongation rates in WS and normal cells.
    • To determine the effect of Simian Virus 40 (SV40) infection on DNA replication in WS cells.

    Main Methods:

    • DNA fiber-autoradiography was employed to measure DNA replication parameters.
    • Experiments were conducted on both infected and uninfected WS and normal cell lines.

    Related Experiment Videos

  • Key metrics analyzed included replicon center-to-center distance and chain elongation rate.

    • Main Results:

    • No significant difference in the rate of DNA chain elongation was observed across all cell types.
    • A significantly longer replicon center-to-center distance was found in WS fibroblasts compared to normal fibroblasts.
    • SV40 infection modulated the replicon center-to-center distance in WS cells, bringing it closer to that of normal cells.

    Conclusions:

    • WS cells exhibit altered DNA replication origin spacing.
    • SV40 infection can influence and potentially normalize DNA replication patterns in WS cells.
    • These findings shed light on replication stress and potential therapeutic avenues in Werner syndrome.