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Structures of ATP(adenosine triphosphate)-vanadyl complexes.

H Sakurai, T Goda, S Shimomura

    Nucleic Acids Symposium Series
    |January 1, 1982
    PubMed
    Summary

    Vanadyl ion forms three distinct complexes with ATP across different pH levels. These findings reveal new insights into vanadium

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    Area of Science:

    • Biochemistry
    • Inorganic Chemistry
    • Biophysical Chemistry

    Background:

    • Cytoplasmic vanadium ions play crucial roles in cellular processes.
    • Vanadyl ion (VO2+) complexes with ATP, and vanadate (VO3-) inhibits (Na++K+)-ATPase.

    Purpose of the Study:

    • To investigate the formation of complexes between ATP and vanadyl ion.
    • To characterize the different types of complexes formed and their coordination environments.

    Main Methods:

    • Potentiometric titration
    • Optical spectroscopy
    • Electron Paramagnetic Resonance (EPR) spectroscopy
    • 31P- and 13C-Nuclear Magnetic Resonance (NMR) spectroscopy

    Main Results:

    • Three types of vanadyl-ATP complexes were observed: a blue 1:1 complex in acidic pH, a blue 1:1 complex in neutral pH, and a green 2:1 complex in alkaline pH.
    • Distinct coordination environments were proposed for each complex type based on spectral data.

    Conclusions:

    • Vanadyl ion forms structurally diverse complexes with ATP, influenced by pH.
    • Understanding these complexes is vital for elucidating vanadium's biological roles and its interaction with ATP.

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