Related Experiment Videos
R46 encodes a site-specific recombination system interchangeable with the resolution function of TnA
Plasmid
|May 1, 1983
Summary
Transposition of the Tn4 element onto the R46 plasmid creates unstable DNA. This leads to rearrangements like deletions and inversions, mediated by a specific recombination system.
Area of Science:
- Molecular Biology
- Genetics
- Microbiology
Background:
- The IncN plasmid R46 is known to interact with transposons.
- Transposon Tn4 is capable of mediating DNA rearrangements.
Purpose of the Study:
- To investigate the DNA rearrangements occurring after transposition of Tn4 onto the R46 plasmid.
- To elucidate the mechanism behind these rearrangements.
Main Methods:
- Analysis of recombinant plasmids formed by R46 and Tn4.
- Characterization of DNA deletions and inversions.
- Investigation of recombination system components.
Main Results:
- Transposition of Tn4 onto R46 generated unstable DNA molecules undergoing further rearrangements.
- Deletions and inversions of R46 and Tn4 sequences were observed, with specific endpoints.
- The rearrangements appear to be mediated by a recA-independent, site-specific recombination system.
Conclusions:
- The study suggests a site-specific recombination system, involving Tn4's cointegrate resolution system and R46-encoded functions, drives these rearrangements.
- R46 appears to encode analogs of Tn4's res site and tnpR gene.
- Models for Tn4-induced deletions and inversions on R46 were proposed.