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Related Experiment Videos

DNA stem-loop structures bind poorly to histone octamer cores.

J Nickol, R G Martin

    Proceedings of the National Academy of Sciences of the United States of America
    |August 1, 1983
    PubMed
    Summary
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    Stem-loop DNA structures do not appear protected within nucleosomes. These DNA structures are found in the spacer regions between nucleosomes or are oriented outward from core particles, remaining accessible to nucleases.

    Area of Science:

    • Molecular Biology
    • Structural Biology
    • Genetics

    Background:

    • Nucleosomes are the fundamental units of DNA packaging in eukaryotes.
    • DNA structure, including stem-loops, can influence nucleosome formation and stability.

    Purpose of the Study:

    • To investigate the structural organization of DNA stem-loop structures within nucleosomes.
    • To determine the accessibility of stem-loop DNA to nucleases when reconstituted into nucleosomes.

    Main Methods:

    • Generation of heteroduplex DNA molecules containing stem-loop structures.
    • Nucleosome reconstitution using heteroduplex and homoduplex DNA.
    • Assessment of DNA accessibility to restriction endonucleases in dinucleosomes.

    Main Results:

    Related Experiment Videos

    • Homoduplex DNA and linear regions of heteroduplex DNA showed significant protection from nuclease digestion.
    • Stem-loop structures within the reconstituted DNA were not protected and remained accessible to nucleases.
    • A significant portion of potential DNA cleavage sites were protected in homoduplexes and linear heteroduplexes.

    Conclusions:

    • Stem-loop DNA structures are preferentially located in the spacer regions between nucleosomes.
    • When incorporated into a nucleosome core particle, stem-loop structures orient outward, maintaining nuclease accessibility.