[Cloning in bacterial and yeast vectors of the hepatitis B virus genome]

Hepatitis B viral genome was cloned simultaneously with bacterial plasmides pBR 325 and pBR 322. Recombinant plasmides were constructed, containing DNA of hepatitis B virus as well as capable to replication in bacterial and yeast cells. Replication of one of these plasmides was possible if it integrated with chromosomal DNA of yeast target-cells; the other plasmides had a capacity to autonomous replication in yeast cells.