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A cryptic plasmid from Shigella sonnei.

J E Croft, P L Bergquist, D Lane

    Journal of General Microbiology
    |May 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    This study characterizes pNZ500, a small cryptic plasmid from Shigella sonnei. It

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    Area of Science:

    • Molecular Biology
    • Microbiology
    • Plasmid Biology

    Background:

    • Shigella sonnei harbors various cryptic plasmids, including the 1.5 kb pNZ500.
    • Understanding plasmid biology is crucial for microbial genetics and biotechnology.

    Purpose of the Study:

    • To characterize the pNZ500 plasmid, including its sequence, maintenance, and gene products.
    • To investigate the relationship of pNZ500 with other known plasmids.

    Main Methods:

    • Introduction of pNZ500 into Escherichia coli via cotransformation.
    • Plasmid copy number determination.
    • Hybridization studies for sequence homology analysis.
    • Investigation of plasmid maintenance requirements (DNA polymerase I activity, protein synthesis).

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  • Analysis of plasmid-encoded polypeptide gene products.

    • Main Results:

    • pNZ500 is maintained at approximately 30 copies per chromosome equivalent in E. coli.
    • High sequence similarity to other 1.5 kb S. sonnei plasmids; limited homology to pBR322 and pAC184 near the ori-bom region.
    • Plasmid maintenance is independent of DNA polymerase I but dependent on protein synthesis.
    • pNZ500 encodes two polypeptides (24.5 kDa and 18 kDa); no distinct plasmid phenotype observed in host cells.

    Conclusions:

    • pNZ500 represents a distinct group of small plasmids within S. sonnei.
    • Its replication mechanism relies on host protein synthesis but not DNA polymerase I.
    • The plasmid does not confer an observable phenotype in E. coli.