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Effect of sucrose phosphate and sorbitol on infectivity of enveloped viruses during storage
Abstract:
Cytomegalovirus and varicella-zoster virus recovery from sucrose phosphate (0.2 M SP) and 70% sorbitol (sorbitol) was compared after storage at -70, 4, and 20 degrees C over time. Recovery from 0.2 M SP was uniformly better. More tissue culture infective doses and infectious foci were recovered in cell monolayers inoculated with 0.2 M SP virus stocks as compared with viruses stored in 70% sorbitol. Although both viruses were isolated from diluted fresh stocks (10(-1) through 10(-4], freezing diluted virus suspensions generally resulted in diminished recovery. Similar stabilizing effects on respiratory syncytial virus and herpes simplex virus type 1 infectivities were observed when stocks were preserved in 0.2 M SP, as compared with 70% sorbitol. Overall, 0.2 M SP was better than 70% sorbitol for stabilizing cytomegalovirus, varicella-zoster virus, respiratory syncytial virus, and herpes simplex virus type 1 infectivities under the conditions tested.
Insights
Sucrose phosphate (0.2 M SP) better stabilizes viruses like cytomegalovirus and varicella-zoster virus than sorbitol during storage. This preservation method enhances virus recovery and infectivity for multiple herpesviruses and respiratory viruses.
Area of Science:
- Virology
- Microbiology
- Biochemistry
Background:
- Accurate virus recovery is crucial for diagnostics and research.
- Standard preservation methods may impact virus viability over time.
- Comparing different storage solutions is essential for optimizing virus stability.
Purpose of the Study:
- To compare the efficacy of 0.2 M sucrose phosphate (SP) and 70% sorbitol in preserving the infectivity of cytomegalovirus (CMV) and varicella-zoster virus (VZV).
- To evaluate the impact of different storage temperatures (-70°C, 4°C, 20°C) and time on virus recovery.
- To assess the broader applicability of 0.2 M SP for stabilizing other viruses, including respiratory syncytial virus (RSV) and herpes simplex virus type 1 (HSV-1).
Main Methods:
- Comparative analysis of virus recovery.
- Storage of CMV and VZV in 0.2 M SP and 70% sorbitol at -70°C, 4°C, and 20°C.
- Quantification of virus infectivity using tissue culture infective doses (TCID) and infectious foci.
- Testing the stabilizing effect on RSV and HSV-1.
Main Results:
- 0.2 M SP consistently yielded better recovery of CMV and VZV compared to 70% sorbitol across all tested conditions.
- Higher numbers of infectious foci and TCID were observed in cell cultures inoculated with viruses stored in 0.2 M SP.
- Freezing diluted virus suspensions generally led to reduced recovery rates.
- 0.2 M SP demonstrated similar stabilizing effects for RSV and HSV-1 infectivity as compared to 70% sorbitol.
Conclusions:
- 0.2 M sucrose phosphate is a superior stabilizing solution for CMV, VZV, RSV, and HSV-1 infectivity compared to 70% sorbitol under the tested storage conditions.
- The findings suggest 0.2 M SP as a more effective medium for preserving viral stocks for research and diagnostic purposes.
- Optimized virus preservation is critical for reliable experimental outcomes and clinical applications.