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Related Experiment Videos

Common multigenic activation in different human neoplasias.

N Hanania, D Shaool, J Harel

    The EMBO Journal
    |January 1, 1983
    PubMed
    Summary
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    A common set of genes, termed tumor-specific DNA (tsDNA), is active in various human cancers. This gene set is largely inactive in normal cells, indicating its potential role in neoplasia development.

    Area of Science:

    • Molecular Biology
    • Oncology
    • Genetics

    Background:

    • A common multigenic component, tumor-specific DNA (tsDNA), was previously identified as transcriptionally active in human lymphoid neoplasias.
    • tsDNA shows minimal activity in normal lymphoid cells, including the Epstein-Barr virus-immortalized Priess cell line.

    Purpose of the Study:

    • To isolate and characterize tumor-specific DNA (tsDNA) from the Raji cell line.
    • To investigate the prevalence of tsDNA transcripts in various human neoplasias and normal tissues.

    Main Methods:

    • Isolation of radioactive Raji cell tsDNA using a recycling procedure to remove common DNA sequences.
    • Utilization of isolated tsDNA as a probe for homologous transcripts.
    • Hybridization of the tsDNA probe with RNAs from diverse human cancer cell lines, patient-derived tumors, and normal cells.

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    Main Results:

    • A significant portion of the tsDNA probe hybridized with RNAs from a wide range of human neoplasias, including leukemias, lymphomas, sarcomas, and carcinomas.
    • Only a minor fraction of the tsDNA probe hybridized with RNAs from non-malignant cells, normal lymphoid cells, fibroblasts, and fetal tissues.
    • The results indicate a conserved multigenic set activated across various human malignancies.

    Conclusions:

    • A common multigenic set is activated in a broad spectrum of human neoplasias.
    • This finding suggests a fundamental molecular mechanism underlying diverse cancer types.