Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Identifying superoxide-sensitive progenitor cells in mouse bone marrow.

W S Chelack, A Petkau

    International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
    |November 1, 1983
    PubMed
    Summary

    A fraction of bone marrow macrophage progenitor cells are sensitive to superoxide radicals. Inactivating this fraction reduces X-ray sensitivity, indicating its role in radiosensitivity.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Comparison of Irradiation and Chemical Fumigation Used in Grain Disinfestation on Production of Ochratoxin A by Aspergillus alutaceus in Treated Barley.

    Journal of food protection·2019
    Same author

    Variants of Aspergillus alutaceus var. alutaceus (formerly Aspergillus ochraceus) with altered ochratoxin A production.

    Applied and environmental microbiology·1991
    Same author

    Role of the competitive microbial flora in the radiation-induced enhancement of ochratoxin production by Aspergillus alutaceus var. alutaceus NRRL 3174.

    Applied and environmental microbiology·1991
    Same author

    Free radicals in health and disease.

    Molecular and cellular biochemistry·1988
    Same author

    Protection of bone marrow progenitor cells by superoxide dismutase.

    Molecular and cellular biochemistry·1988
    Same author

    Enrichment of progenitor cells from human bone marrow by flow cytometry.

    Molecular and cellular biochemistry·1988

    Area of Science:

    • Cell Biology
    • Radiation Biology
    • Hematopoiesis

    Background:

    • Macrophage progenitor cells are crucial for immune function and hematopoiesis.
    • Cellular sensitivity to oxidative stress and radiation varies.
    • Superoxide radicals are reactive oxygen species implicated in cellular damage.

    Purpose of the Study:

    • To investigate the role of superoxide-sensitive cells within macrophage progenitor populations.
    • To determine the relationship between superoxide sensitivity and X-ray radiosensitivity in these cells.

    Main Methods:

    • Culturing bone marrow macrophage progenitor cells in liquid medium.
    • Generating superoxide radicals photochemically to assess cell sensitivity.
    • Irradiating cells with X-rays to measure radiosensitivity.

    Related Experiment Videos

  • Comparing radiosensitivity between normal and photochemically inactivated cell populations.

    • Main Results:

    • A variable fraction of macrophage progenitor cells exhibited sensitivity to photochemically generated superoxide radicals.
    • Cell populations with a higher superoxide-sensitive fraction demonstrated increased sensitivity to X-ray irradiation.
    • The observed change in radiosensitivity was directly proportional to the proportion of superoxide-sensitive cells.

    Conclusions:

    • Superoxide-sensitive cells within macrophage progenitor populations significantly influence cellular radiosensitivity.
    • Targeting or understanding these superoxide-sensitive cells may have implications for radiation therapy or understanding radiation damage.