Hydrocortisone and isoproterenol effects on trachealis cAMP and relaxation

Isoproterenol concentration-response curves for cAMP formation and relaxation were determined in control and hydrocortisone-treated strips of canine tracheal smooth muscle. Adenosine 3'5'-cyclic monophosphate(cAMP) formation and muscle relaxation were well correlated, and both responses were enhanced proportionally by hydrocortisone treatment. Guanosine 3'5'-cyclic monophosphate was unchanged by isoproterenol but was increased to a small but significant extent by hydrocortisone. Prostaglandin E2 (not a beta-adrenergic agonist) relaxed the muscle strips, but this effect was not enhanced by hydrocortisone pretreatment. Our data are compatible with the concept that cAMP is an obligatory intermediate in the chain of events by which beta-adrenergic agonists relax airway smooth muscle. The action of hydrocortisone on this process is localized at or before cAMP formation, since it enhanced both cAMP formation and relaxation to the same extent.