T cell clone-specific alloantisera that inhibit or stimulate antigen-induced T cell activation
Journal of Immunology (Baltimore, Md. : 1950)
|December 1, 1983
Summary
Researchers generated antibodies targeting T cell receptors (TCRs) on T cell hybrids. These antibodies specifically inhibited antigen-induced IL 2 secretion, confirming their reactivity with the T cell antigen receptor.
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- T cell hybrids secreting Interleukin-2 (IL-2) are valuable tools in immunology research.
- Understanding the specificity of T cell antigen receptors (TCRs) is crucial for immune response studies.
Purpose of the Study:
- To generate alloantibodies against the TCRs of T cell hybrids.
- To characterize the specificity and function of these generated antibodies.
Main Methods:
- Immunization of F1 mice strains with T cell hybrids (2B4 and 2C2).
- Generation of malignant ascites containing antibodies.
- Assays to measure inhibition or synergy of antigen-induced IL-2 secretion.
Main Results:
- Ascites from mice immunized with 2B4 T cell hybrid contained antibodies that specifically inhibited antigen-induced IL-2 secretion from 2B4 cells.
- Antibodies from 2C2 immunization inhibited IL-2 secretion from 2C2 cells at high concentrations and synergized at low concentrations.
- Concanavalin A-induced IL-2 release was unaffected, indicating antigen-specific TCR interaction.
Conclusions:
- The generated ascites contain antibodies that specifically bind to the antigen-specific TCR on T cell hybrids.
- These antibodies can be used to study TCR function and specificity.
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