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Related Experiment Videos

A Bodian method for mounted frozen sections.

B E Herr, P D Coleman, R C Griggs

    Stain Technology
    |September 1, 1976
    PubMed
    Summary
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    The Bodian method can be reliably applied to frozen nerve and muscle sections, producing results comparable to traditional paraffin embedding techniques. This simplified frozen section protocol minimizes artifacts and allows for versatile serial staining options.

    Area of Science:

    • Neuroscience
    • Histology
    • Biomedical research

    Background:

    • The Bodian method is a crucial technique for neuroanatomical studies.
    • Traditional application requires paraffin embedding, which can be time-consuming and introduce artifacts.
    • Adapting the Bodian method for frozen sections could offer a more efficient and artifact-minimized alternative.

    Purpose of the Study:

    • To adapt and validate the Bodian staining method for use with frozen tissue sections.
    • To compare the efficacy of the Bodian stain on frozen sections versus paraffin sections.
    • To assess the simplicity, reliability, and artifact level of the modified technique.

    Main Methods:

    • Peripheral nerve or muscle tissues were sectioned at 10 µm (micrometers) while frozen.

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  • Tissues were fixed in a mixture of 80% ethanol, 10% formalin, and glacial acetic acid.
  • Central nervous tissue underwent fixation before and after freezing and mounting.
  • Sections were stained using the standard Bodian impregnation procedure.

    • Main Results:

    • The Bodian stain applied to frozen sections yielded results comparable to those obtained from paraffin-embedded sections.
    • The modified technique demonstrated simplicity and reliability for both peripheral and central nervous tissues.
    • Utilizing 10 µm frozen sections resulted in minimal artifacts.

    Conclusions:

    • The Bodian method is effectively applicable to frozen tissue sections for histological staining.
    • This frozen section protocol is a simple, reliable alternative to paraffin embedding, offering comparable staining quality.
    • The technique preserves tissue integrity, minimizes artifacts, and allows for parallel staining with other methods on serial sections.