Retinal pigment epithelium in culture demonstrates a distinct beta-adrenergic receptor

Retinal pigment epithelial (RPE) cells in culture, as well as freshly dissected embryonic RPE, exhibit a beta-adrenergic receptor. Agonist potency is: L-isoproterenol greater than (-)epinephrine greater than or equal to (-)norepinephrine much greater than dopamine. Propranolol blocks this effect. Hydroxybenzylpindolol (HYP), a beta-antagonist, also blocks the increase in cAMP elicited by L-isoproterenol. Binding of [125I]-hydroxybenzylpindolol, shows a half saturation concentration of 1.1 X 10(-9) M and 2.7 X 10(5) HYP binding sites per cell. The presence of such distinct beta-adrenergic responsiveness indicates a potentially important role for this receptor in RPE cell physiology.